A flow Cytometry Gating Strategy for Profiling CD4+CD25+/highCD127-/low Regulatory T Cells in the Rheumatoid Arthritis Scenario

Authors

  • Martínez Rosales Ricardo Ulises
  • Crespo Oliva Celia del Carmen
  • Chávez Valdés Sheila
  • Vazquez Arteaga Amalia
  • Coizeau Rodríguez Edelgis
  • Ávila Díaz Lismary
  • Freyre Corrales Giselle
  • Gonzalez Formental Hany Lianet
  • Chacón Quintero Yahima
  • Lemos Perez Gilda

Abstract

Regulatory T cells (Tregs) has been consider as a potential biomarker in rheumatologic clinical trials as this cell population has been shown to be involved in the autoimmune diseases outcome. Human Tregs are defined as CD4+CD25+/highCD127-/low cells; however its accurate identification by flow cytometry has been argued, especially as a result of different gating strategies. A flow cytometry gating strategy was settled to measure human Tregs from PBMC isolated via density gradient using CPT tubes. With this method we performed a standardized procedure for Treg profiling, useful for immunomonitoring in clinicals assessment.

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Published

2023-05-10

Issue

Vol. 6 No. 2 (2023): Volume 6, Issue 2

Section

Articles